Fig 1: Melting curves of PCR products of CFTR, ATP6V0D2, ATP6V1C2, and β-actin mRNAs.
Fig 2: Relative expression of CFTR, ATP6V0D2, and ATP6V1C2 mRNA in yak lungs at different ages. A. Relative expression of CFTR mRNA in the yak lungs. B. Relative expression of ATP6V0D2 mRNA in the yak lungs. C. Relative expression of ATP6V1C2 mRNA in the yak lungs. Note: Different lowercase letters indicate significant differences (p < 0.05), and the same letters indicate non-significant differences (p > 0.05).
Fig 3: Relative Expression of CFTR, ATP6V0D2, and ATP6V1C2 proteins in yak lungs at different ages. (A) Representative images of Western blotting results. (B) Analyses of the gray values of CFTR. (C) Analyses of the gray values of ATP6V0D2. (D) Analyses of the gray values of ATP6V1C2. Note: 1. Newborn group; 2. Juvenile group; 3. Adult group; and 4. Elderly group. Different letters represent statistically significant differences (p < 0.05).
Fig 4: Results of conventional PCR amplification of β-actin, CFTR, ATP6V0D2, and ATP6V1C2 mRNAs. 1. Newborn group; 2. Juvenile group; 3. Adult group; and 4. Elderly group.
Fig 5: ATP6V1C2 localization in yak lungs at different ages. (a) Small bronchi of yak lungs in the newborn group (200×). (b) Small bronchi and pulmonary arteries of yak lungs in the newborn group (200×). (c) Bronchioles of yak lungs in the juvenile group (400×). (d) Bronchioles and pulmonary arteries of yak lungs in the juvenile group (400×). (e) Bronchioles of yak lungs in the adult group (400×). (f) Pulmonary arteries of yak lungs in the adult group (400×). (g) Bronchioles and pulmonary arteries of yak lungs in the elderly group (400×). (h) Terminal bronchioles of yak lungs in the elderly group (400×). (i) Blank control of small bronchi and pulmonary arteries of yak lungs in the newborn group (200×). (j) Blank control of bronchioles and pulmonary arteries of yak lungs in the juvenile group (200×). (k) Blank control of bronchioles of yak lungs in the adult group (200×). (l) Blank control of bronchioles of yak lungs in the elderly group (200×). SB—small bronchi; B—bronchiole; TB—terminal bronchiole; PA—pulmonary artery.
Supplier Page from Abcam for Anti-ATP6V1C2 antibody